ALOX15 ELISA Kit

Catalog No. ABIN2042937

This Human ALOX15 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human ALOX15.

Quick Overview for ALOX15 ELISA Kit (ABIN2042937)

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details ALOX15 ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Brand: BioAssay™

Sensitivity: < 0.57 ng/mL

Application Details

Plate: Pre-coated

Protocol: Arachidonate-15-Lipoxygenase (ALOX15) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-15-Lipoxygenase (ALOX15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-15-Lipoxygenase (ALOX15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-15-Lipoxygenase (ALOX15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arachidonate-15-Lipoxygenase (ALOX15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Alternative Name: Arachidonate-15-Lipoxygenase (ALOX15)

Pathways: Regulation of Actin Filament Polymerization